3500xl genetic analyzer manual

This manual contains protocols for use of the PowerPlex and xL Genetic Analyzers. Protocols to operate the fluorescence-detection instruments should be obtained from the instrument manufacturer. Amplification and detection instrumentation may vary. You may need to optimize protocols including amount of template DNA, cycle number. We set a new standard in capillary electrophoresis with the 8-capillary and capillary xL Genetic Analyzers. The 8-capillary platform can run a wide variety of applications —including de novo sequencing and resequencing (mutational profiling)—as well as microsatellite analysis, MLPA™, LOH, MLST, AFLP® and SNP validation or screening. GeneMarker® v Operation Manual 1 Guiding Principles and Scope This method contains the steps for the analysis of PowerPlex® Fusion 5C (Fusion) and PowerPlex® Y23 (PPY23) STR runs performed on xL Genetic Analyzers and analysis of PowerPlex® Fusion 5C (Fusion) and AmpFlSTR® Yfiler (Yfiler) STR runs performed on.

The capillary xL Genetic Analyzer sets the standard in capillary electrophoresis. The series instruments are the first genetic analyzers designed with a specific feature set and workflow for the Human Identification application. The complete product combines the instrument with Applied Biosystems™ reagents, consumables and. Applied Biosystems User Manual. For Research Use Only. Not for use in diagnostic procedures. Page 1 ™ Applied Biosystems /xL Genetic Analyzer USER GUIDE with Series Data Collection Software Catalog Numbers , Publication Number Revision C For Research Use Only. DNA Procedures Manual nDNA__nDNA_PostAmp Issue Date: 12/30/ Revision: 8 Page 2 of 18 xl Instrument Reagents: o 1X Genetic analyzer buffer with EDTA, Applied Biosystems or equivalent. o Performance Optimized Polymer 4 (POP-4™), Applied Biosystems xL Instrument Reagents.

Key features of the Series genetic analyzers We have enhanced our gold-standard Series instruments and GeneMapper ID-X software solutions in response to customers' need to decrease data analysis time, increase confidence in their results, allow for a more efficient processing workflow, and comply with ever-changing IT requirements. ABI PRISM ® and Avant Genetic Analyzers and Applied Biosystems , xl, and xL Genetic Analyzers (Figure 1). Protocols to operate the fluorescence-detection instruments should be obtained from the instrument manufacturer. Amplification and detection instrumentation may vary. You may need to optimize protocols. Genetic Analyzers. The IGT 5-Dye Matrix Standard consists of DNA fragments labeled with five different fluorescent dyes, FAM, JOE, TMR, ROX, and TGI-ORANGE. These matrix fragments are used on the Applied Biosystems , xL, or xL Genetic Analyzer to perform a spectral calibration on a specified dye set.